― Advice in development, validation, setting up Quality and reporting system of new micro sampling assays
IIn 2006 we started developing a Dried Blood Spot (DBS) method in the Maastricht Academic hospital MUMC+ for Tacrolimus. [Hoogtanders K van der Heijden J, et al: J. Pharm Biomed Anal: 44 (2006): 658 – 664]. After extensive validation, the method was used for different projects like comparison of venous sampling versus micro-sampling and a large clinical trial for testing a delayed release drug. [Frank Stifft et al: Transplantation 2013;00: 00Y00]. Micro-blood sampling made this study much more costs effective.
In 2013 we founded DBSL, a company specialized in micro sampling. We were the first company in developing a Vitamin D3 micro sampling assay with the possibility to determine the 3-epi isomer of 25(OH)D3 for measurement in children between 0.5 and 4 years.
Using our Vitamin D3 analysis based on micro-blood sampling, we did several projects like Vitamin D3 assessment of more than a thousand individuals visiting the Nation Wide Radio Action Serious Request 2015 as well assessment of large numbers of health care employees participating in several symposiums.
― Advice in conversion of manual sample preparations to a high throughput for micro-sampling analyses
Clinical laboratories are well equipped to process large numbers of venous blood samples almost fully automatically. At this stage automation of clinical chemical analyses is often not suitable for micro-sampling Automatization depending on the method of micro sampling technique will however become feasible in the near future. I have set up micro-sampling methods for LC-MS/MS and immuno-analyzers (HbA1c). Currently I am working on automatization of Vitamin assays using magnetic beads.
― Advice/Guidance in implementation of analyses in micro sampling from sample request to validated result report
Although micro-sampling is gaining more and more attention, acceptance is not always there. Clear instructions to the person who has to take the sample is imperative in order to obtain useful blood samples . In DBSL we took care of the entire chain of custody from micro sampling instruction to reporting of validated results.
- No para(medical) intervention
- Less invasive
- Everywhere applicable
- Blood sampling at any time (fasting)
- Easy transport by post (courier)
- Uncomplicated storage